arXiv:2507.17518v1 Announce Type: cross Abstract: Digital Twins (DTs) are gaining prominence in cybersecurity for their ability to replicate complex IT (Information Technology), OT (Operational Technology), and IoT (Internet of Things) infrastructures, allowing for real time monitoring, threat analysis, and system simulation. This study investigates how integrating DTs with penetration testing tools and Large Language Models (LLMs) can enhance cybersecurity education and operational readiness. By simulating realistic cyber environments, this approach offers a practical, interactive framework for exploring vulnerabilities and defensive strategies. At the core of this research is the Red Team Knife (RTK), a custom penetration testing toolkit aligned with the Cyber Kill Chain model. RTK is designed to guide learners through key phases of cyberattacks, including reconnaissance, exploitation, and response within a DT powered ecosystem. The incorporation of Large Language Models (LLMs) further enriches the experience by providing intelligent, real-time feedback, natural language threat explanations, and adaptive learning support during training exercises. This combined DT LLM framework is currently being piloted in academic settings to develop hands on skills in vulnerability assessment, threat detection, and security operations. Initial findings suggest that the integration significantly improves the effectiveness and relevance of cybersecurity training, bridging the gap between theoretical knowledge and real-world application. Ultimately, the research demonstrates how DTs and LLMs together can transform cybersecurity education to meet evolving industry demands.