Today we’re joined by Jianxu Chen, a scientist at the Allen Institute for Cell Science. At the latest GTC conference, Jianxu presented his work on the Allen Cell Explorer Toolkit, an open-source project that allows users to do 3D segmentation of intracellular structures in fluorescence microscope images at high resolutions, making the images more accessible for data analysis. We discuss three of the major components of the toolkit: the cell image analyzer, the image generator, and the image visualizer